GBIO 434/534
Molecular Biology and Biotechnology
Quiz 1
True/False
1) The earliest manipulation of nature for human benefit was creation of recombinant DNA
technology.
False
2) Molecular Biotechnology arose out of an increased understanding of Genetics and
Molecular Biology.
True
3) Advances in knowledge in Genetics and Molecular Biology that helped lead to Molecular
Biotechnology included studies pre 1920 by Griffith/Avery/Hershey/Chase, Chargaff,
Franklin/Wilkins/Watson/Crick, and Cohen and post 1920 by Mendel, Darwin/Wallace,
Meischner, Sutton/Boveri, and Morgan.
False
4) Genetically Modified Organism crop plants could contain genes for insect resistance (Bt),
herbicide tolerance (Ht), drought resistance, or disease resistance.
True
5) Restriction enzymes are endonucleases that always leave overhangs (staggered ends).
False
6) The natural source of restriction enzymes is eukaryotic cells and their natural targets are
viruses that infect eukaryotic cells.
False
7) Recombinant DNA technology can be as simple as creating a plasmid using a restriction
enzyme to cut at a restriction site in the polylinker (cloning site) of the plasmid, cutting
out a gene from a larger DNA fragment using the same restriction enzyme, and using
DNA ligase to paste the gene into the plasmid.
True
8) “Competent” cells (bacteria), have been treated to make restriction enzymes.
False
9) Transformation can be used to describe the introduction of a plasmid into bacteria.
Selection of bacteria that carry the plasmid involves growth of colonies (cloning) on agar
