Lab 4- equillibrium constants, Lab Reports of Chemistry

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Iinformati Mame: 250 mL Flask Volume: 220,000 mL Species (aq) Molarity : - He 1LO0885e-7 | cr ‘LOOBESe-7 ir 0.00163844 co a 000163844 350 mi, aT 00483616 20,000 ml, Temperature: 25.1190 pH: 7.00 6. Make calculations about binding of the bases ta form the base pair. The binding reaction equation is A+7T 3 AT The equilibrium constant for this reaction is referred to as the “binding constant” for A and T, and AGe for the reaction is the “free energy of binding”. Determine the equilibrium constant and the free energy of the binding reaction. And show the calculation work in Data Sheet. 7. Design an experiment to determine the equilibrium constant and the free energy of binding reaction of C and G ‘to form GG. Hint: repeal Step 1-6, except replace A with C, and T with G. Record the equilibrium conaentrations of C, G and GG in the Data Sheet, Table 2. Determine the equilibrium cometant and the free energy of lhe binding reaction babween C and G. And show the calculation work in Data Sheet. &. Design an experiment to determine the equilibrium constant and the free energy of binding reaction of & and C to form a mis-match pair AC, Hint: repeat Step 1-6, except replace C with T, Record the equilibrium ‘concentrations of A, C and AC in the Data Sheet, Table 2. Determine the equilibraim constant and the free energy of the binding reaction between A and, And shew the calculation work in Data Sheet, Data Sheet Table 1: AT binding. The chemical equation is_A + T > AT [Alea | Tao TAT] oo a Temperature in °C | Temperature in K (lo35A > 1u35H c= |Ygset ee | 95° q4¢ 7 ss: \$,067-7 K(binding of AT) = tt. = 1. 63 sa4e73 [legl Fle = \ AG® (binding of AT) =-RT in k= #3 M246) In (180577) = - A495 Ame R is the gas constant 8.31 J/imol.K; T is the temperature in K.