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Exam 2 Questions for Molecular Genetics - Fall 2006 | BI 381, Exams of Molecular biology

Material Type: Exam; Professor: Gathman; Class: Molecular Genetics; Subject: Biology; University: Southeast Missouri State University; Term: Spring 2006;

Typology: Exams

Pre 2010

Uploaded on 08/08/2009

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BI 381 Exam 2 Spring 2006
Instructions: 1. Relax. 2. Genetic code and wobble tables at the end of the exam.
a. (20 points) In the table below, indicate whether each mutant organism will be able to
grow when placed on each medium by putting + for growth of the organism and - for no
growth of the organism. Please make them clear so I can read them.
Note: Compound B is hydrolyzed to yield D and E; compounds C and D are combined
by condensation to form F; compound G and some compound included in minimal
medium are combined by condensation to form H. The organism must either make or
receive compound J in order to grow. All mutants are auxotrophs, and each is mutant for
only one gene (coding for one enzyme).
Minimal Medium Supplemented with Compound
Mutant (none) A B C D E F G H I J
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2
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pf3
pf4
pf5

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BI 381 Exam 2 Spring 2006 Instructions: 1. Relax. 2. Genetic code and wobble tables at the end of the exam. a. (20 points) In the table below, indicate whether each mutant organism will be able to grow when placed on each medium by putting + for growth of the organism and - for no growth of the organism. Please make them clear so I can read them. Note: Compound B is hydrolyzed to yield D and E; compounds C and D are combined by condensation to form F; compound G and some compound included in minimal medium are combined by condensation to form H. The organism must either make or receive compound J in order to grow. All mutants are auxotrophs, and each is mutant for only one gene (coding for one enzyme). Minimal Medium Supplemented with Compound Mutant (none) A B C D E F G H I J 1 2 3 4 5 6 7 8 9

  1. a. (10 points) Describe the process of intron removal and exon splicing in a eukaryotic RNA transcript. Be as specific as possible about the snurps involved and the sequence of events. b. (10 points) Describe the process of polyadenylation of a eukaryotic RNA transcript. Be as specific as possible about the enzymes involved and the sequence of events.
  1. (5 points each) For each of the following types of lesions in E. coli , tell whether it would be most likely to be repaired by direct reversal, base excision repair, mismatch repair, or nucleotide excision repair, and justify your answer. a. spontaneous deamination of cytosine results in a uracil in the DNA b. X-ray exposure causes breakage of bonds in a protein. This results in a tripeptide being added to a guanine in the DNA. c. UV-induced thymine dimer
  1. Mutations in the XPD gene in mice cause premature aging. The XPD protein is known to function in denaturing DNA in both transcription and NER, so XPD mutants have partial defects in both transcription and NER. XPA mutants show a complete loss of NER function. a. (5 points) deBoer et al. proposed two hypotheses to explain the premature aging in XPD mutant mice. What were those hypotheses? b. (10 points) What did each hypothesis predict would be observed in XPD/XPA double mutants, compared to XPD mutants? Explain why each hypothesis predicts what it does.