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Key concepts and questions related to essential microbiology, focusing on microscopy and staining techniques. It provides an overview of topics such as the definition of a nanometer, the relationship between resolution and contrast, the use of microscope components to control light, the principles of different microscopy techniques (e.g., fluorescence, phase-contrast, dark field, confocal), the interpretation of electron microscopy images, and the application of gram staining and other staining methods. Likely intended as study material or exam preparation for a university-level microbiology course, covering fundamental knowledge and skills required for understanding and working with microscopic biological samples.
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Question 1
. A nanometer is defined as: A. 10 -^3 B. 10 -^6 C.10-^9 D. 10 -^12 C. A nanometer is defined as one-billionth of a meter.
A. Objective B. Condenser C. Iris diaphragm D. Eye piece C. The iris controls the amount of light that passes through the sample and into the objectivelens. Thus, it can be adjusted (opened or closed) to alter the amount of light.
The above image is captured via a Transmission Electron Microscope (TEM). Even at 20nmresolution (inset image), subcellular substructures are still visible. The image lacks the outside ‘shell’ only appearance of SEM.