Name:_________________________________Section:_______Date:______________
Bio 114
Enzyme Lab
Data Sheet
10 pts.
*Note: You must wear goggles for this lab! Your lab instructor will provide goggles
for you to wear during this lab.
Fill in the following table and answer the questions below (1 pt. each):
Titration Tube #1 (on ice) Tube #2 (room temp.)
Beginning buret volume ml ml
Ending buret volume ml ml
Amount required to titrate
(ending vol. – beginning vol.)
ml ml
1. Which tube had more substrate remaining (i.e., which tube require more KmnO4 to
titrate)?___________________________________
2. In which tube did the reaction proceed at a faster rate?__________________________
3. Why did tube #1 and tube #2 have different rates of reaction (i.e., what caused this to
happen)?_____________________________________________________________
4. What would happen if we had incubated a tube containing 3 ml of hydrogen peroxide
+ 10 drops of catalase at 100C (boiling)? Remember that enzymes are proteins!
__________________________________
