Creative Biolabs Released TCR Repertoire Sequencing Service Based on Its MagicTM
Platform
Creative Biolabs released TCR repertoire sequencing service with the MagicTM platform
Based on its MagicTM Platform, scientists from Creative Biolabs has developed MagicTM TCR
repertoire sequencing service, which can sequence CDR regions of the whole four types of TCR
chains , α, β, γ, and δ.
By integrating the next generation sequencing (NGS) technology and other specific techniques
into the MagicTM platform, Creative Biolabs helps in increasing the high-throughput sequencing
capacity. Apart from large-scale sequencing, this platform can also be specially designed for
certain service. The MagicTM IR-seq platform allows RNA from human or mice as a template to
amplify and sequence the CDR of whether α, β, γ or δ chain of TCR. Researchers can obtain the
sequence data of whole covered CDR 3 regions of the interested TCR chain from either human or
mice which involves most of the hypermutation information.
The effective TCR repertoire is quite important in the adaptive immune system. TCR locating on
the T-cell surface is responsible for interacting with peptide fragments of antigens that bound to
MHC molecules. Generally, most of the TCRs consist of an α chain and a β chain as a heterodimer,
and a minority of them are formed by a γ chain and a δ chain as an alternative. α/β TCR
accounting for 95% and γ/δ TCR 5% for a healthy human. However, the proportion between α/β
TCR and γ/δ TCR is different due to ontogenesis, disease influences or different species.
Similar to half of the Fab fragment of an antibody, each TCR chain is composed of a constant and
a variable region which has also three complementarity determining regions (CDRs). Meanwhile,
the TCR α and γ chain are generated by VJ recombination just like the light chain of an antibody,
and the β chain and δ chain undergo VDJ recombination which is similar to the heavy chain of an
antibody. It is during this rearrangement mechanism that the nucleotide additions or deletions at
the junctions endow the TCR repertoire with essential diversity.
“In this way, the sequencing and analysis of TCR repertoire is a meaningful direction to enhance
the understanding of the immune system”, said Dr. Monika Müller, scientific officer of Creative
Biolabs, “especially under different stimulations such as infections, autoimmune diseases and
cancers”.
About Creative Biolabs
Creative Biolabs is specialized in providing custom biotechnology and pharmaceutical services
that cover the full scope of biotechnology needs of early drug discovery and development.
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