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Biology Lab 12 Biology lab, Lab Reports of Chemistry

Biology/Chemistry Laboratory homework for Lab 12

Typology: Lab Reports

2021/2022

Uploaded on 04/24/2023

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bg1
synapomorphies include a
complete
digestive
system
(runs
from
mouth
to
anus),
a double ventral
nerve
chord,
and
excretory
organs
called
the
Maphighian
tubes.
Sexes
are
separate:
arthropods
copulate
and
have
internal
fertilization.
Most
arthropods
care
for
the
eggs
in
some
way,
either
by
laying
eggs
with
protective
shells
in
or
on
a
food
source or
by
carrying
them
around
on
their
bodies
until
the
eggs
hatch.
Females
carrying
eggs
are
called
gravid
females.
Larvae
are
often
very different
from
adults
and
undergo
metamorphosis
in
a short
period
of
time.
ACTIVITY
1:
Arthropod Dissection
In
this
laboratory,
you
will
be
working
in
groups
of
two
on
the
dissection of
the
organism assigned
to
you
by
the
professor.
You
will
be
responsible
for identifying
the
external
and
internal features of your
organism
using
the
Photographic
Atlas
and
other
resources
as
provided
by
your instructor.
(NOTE:
Don't forget
to
check
out
the
other specimens
that
are
available
at
the
rear of
the
lab.)
Exercise
1:
Anatomy
of Crayfisb
As
a
member
of
the
Class
Crustacea,
the
crayfish
shares synapomorphies with other crustaceans.
This
includes
jointed,
paired
,
and
biramous
appendages,
two
pairs
of antennae,
and
three body
regions
(the
head,
thorax,
and
abdomen).
Crayfish
are
known
to
be
scavengers, but they
also
prey
ion
other invertebrates. The
mouth
is
surrounded
by
appendages modified for feeding,
and
there is a
well-developed digestive
tract.
Dorsal,
anterior,
and
posterior arteries carry hemolymph (blood plus
lymph)
to tissue spaces (hemocoel)
and
sinuses.
In
contrast
to
vertebrates, there is a ventral solid
nerve
cord.
Crayfish
are
adapted
to
an
aquatic existence.
External
Anatomy
148
1.
Obtain a preserved crayfish,
and
place
it
in
a dissecting
pan
.
You
will draw
the
exterior
and
interior of
the
crayfish
in
the
boxes
following
the
dissection instructions. Make sure
to
label
the balded structures
on
your drawings
as
you
go
though the dissection.
2. Identify
the
chitinous exoskeleton.
3.
Arthropods
are
distinguished
by
metameres (repeating body segments) that fuse
to
form
different body regions (tagmata;
tagma
sigular). There
are
two tagmata
in
the crayfish.
The
first
is
the
cephalothorax
(fusion of the
head
and
the
thorax),
which
is
covered
by
a
carapace.
The second
is
the
abdomen,
which consists of several metameres that go
all
the
way
down
to
the
tail.
Q:
Is
each
region
specialized for
its
function? -~
-+r
e..:
~5
___________
_
Q:
Which body
region
of the crayfish
is
most obviously segmented?
B
rvleN\e
~
4.
Find
the
antennae,
which project
from
the head. Crustaceans also contain a secondary
set of smaller antennae
(antennule).
At the base of
each
antenna, locate a small,
pf3
pf4
pf5
pf8
pf9
pfa

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synapomorphies include a complete digestivesystem (runs from mouth^ to^ anus),a double ventral nerve chord, andexcretory organs called theMaphighian tubes.

Sexesareseparate:^ arthropods^ copulateandhaveinternal^ fertilization.^ Most^ arthropods^ care^ for^ the eggsinsomeway,eitherbylaying eggs withprotective shellsinoronafoodsource or by carrying them aroundontheir^ bodiesuntil^ the^ eggs^ hatch.^ Females^ carrying^ eggsarecalled^ gravid^ females. Larvae are oftenvery differentfrom adultsandundergo metamorphosisina short period oftime.

ACTIVITY1:Arthropod Dissection

Inthis laboratory,you willbeworkingingroups^ oftwoonthedissection of^ the^ organism assigned^ to youbytheprofessor.You willberesponsible for identifying the externalandinternal features of your

organism using the Photographic Atlasandother resourcesasprovided by your instructor.^ (NOTE:

Don't forgettocheck out the other specimens that are available attherear of^ the^ lab.)

Exercise1:Anatomy of Crayfisb

Asa member of^ the^ Class^ Crustacea,^ the^ crayfish^ shares synapomorphies with other crustaceans. This includes^ jointed,^ paired^ ,andbiramous^ appendages,^ two^ pairs^ of antennae,andthree body regions (the head, thorax, and abdomen). Crayfishareknownto bescavengers, but they^ also^ prey ion other invertebrates. The mouthissurrounded by appendages modified for feeding,andthere is a well-developed digestive tract. Dorsal, anterior,andposterior arteries carry hemolymph (blood plus lymph) to tissue spaces (hemocoel)andsinuses.Incontrasttovertebrates, there is a ventral solid nerve cord. Crayfish are adapted toanaquatic existence.

External Anatomy

  1. Obtain a preserved crayfish, and place itina dissecting pan. You will draw the exterior and interior of the crayfishinthe boxes following the dissection instructions. Make sure to label the balded structuresonyour drawings^ as^ you^ go^ though the dissection.

2. Identify the chitinous exoskeleton.

  1. Arthropods^ are^ distinguished^ by^ metameres (repeating body segments) that fuse^ to^ form different body regions (tagmata;^ tagma^ sigular).^ There^ are^ two tagmatainthe crayfish.

The firstisthe cephalothorax (fusion of the^ head^ and^ the^ thorax),^ which^ is^ covered^ by

a carapace. The second is the abdomen, which consists of several metameres that go

all the way down^ to^ the^ tail. Q: Iseach region specialized for its function? -~-+re..:~5 ___________ _

Q: Which body region of the crayfish^ is^ most obviously segmented? BrvleN\e ~

4. Find the antennae, which project from the head. Crustaceans also contain a secondary

set of smaller antennae (antennule). At the base of each^ antenna, locate a small,

raisednipple containinganopeningforthe^ green glands,^ theorgans^ of^ excretion. Crayfish excretealiquid^ nitrogenouswaste. Q:Thenitrogenous waste would result fromthebreakdownof^ which^ group^ of macromolecules? pro.t-...:.n:S ~ /'\LAc.l£c..i,^ <:U:c.l

  1. Locatethe compound eyes, whicharecomposed^ ofmanyindividual^ units^ (ommatidia) forsight.Identifythe^ rostrum,^ theprojection ofthecarapace^ above^ the^ eyes.
  2. Identifythe sixpairs of feeding appendages^ around^ the^ mouthfor handlingfood.
  3. Find the five pairs of appendages attachedtothecephalothorax.^ The^ most anterior^ pair isthepincer-like claws (chelipeds).^ Theother four^ pairs^ are^ walking legs. Q: How many appendagesarechelate (thatis, pincer-likewithopposing^ claws)?^ ·^3
  4. Locatethefive pairs of swimmerets^ onthe^ abdomen.Inthemale,^ theanteriortwopairs arestiffenedandfolded^ forward.^ They^ are^ copulatory swimmerets^ and^ aidinthe transfer of sperm during mating. Q: Are theanterior swimmerets different^ from^ the^ posterior^ pair?^ - v U. ~-=-^ (:!:^5 -----
  5. In the female,^ identify^ the^ seminal receptacles,^ a swelling located^ between^ the^ bases^ of the thirdandfourth pairs of walking^ legs.^ Sperm^ from^ the^ male^ are^ depositedinthe seminal receptacles.Inthe^ male,^ identify the opening ofthe^ sperm^ duct located^ at^ the base of the fifth walking^ leg^ .Inaddition,oneither side of the middle walking^ legs^ are^ two openings, which lead to the oviduct. The^ eggs^ are^ released^ onto^ the^ exterior^ region between^ the^ walking^ legs,^ allowing for external fertilization.^68 Q: What sex is your specimen? t.::....e=--..c~- -:...^ ______________^ _ (Make sure to view a crayfish of^ the^ opposite sex^ and^ note^ the^ differences). Q: How can^ you^ differentiate a male crayfish from a female crayfish? ~)(.\m- ~\MM-u-^ (<U-S^ ~^ o.Q.^ tairt^ O('d.^ f~~=

Q: How would you differentiate walking legs from swimmerets? WO-\.~~ ~~ 0-("J!^ a~4-5-es^ ~^ dt-d;^ :tc

  1. Find the last abdominal segment, which bears a pair of^ broad,^ fan-shaped^ uropods, which, together with a terminal extension of the body (the^ telson),^ form a^ tail. Q: ~ oyt does the structure of the uropods correlate^ to^ its function? rw (M))0Jd:S^ (.,.X,c(^ [c,'el¼,,^ -^ lVI~^ :!k^ h.l'=>ol'c^ ~"-^ oede.r

(^68) Mac0rganisms2: Crayfish Anatomy; https://www .youtube.com/watch?v=2cBGuEDxvNo

I I I J I - , ,

  1. Identify the rhomboid-shaped heart lying in the mid-dorsal region. A crayfish has^ an

open circulatory system. Carefully remove the heart. Note the openings (ostia).

  1. Locate the gonads anterior to the heartinboth the male and female. The gonads are

tubular structures bilaterally arranged in front^ of^ the heart and continuing behind it as a single mass.Inthe male, the testes are highly coiled, white tubes.

  1. Find the mouth; the short, tubular esophagus; and the two-part^ stomach,^ with

attached digestive gland, that precedes the intestine.

  1. Identify the green glands, two excretory structures just anterior to the stomach,^ on

the ventral segment wall. They are generally darkincolor.

  1. Remove the thoracic contents previously identified. 11. Identify the brain infront of the esophagus. The brain is very small and may be

difficult to see. It is connected to the ventral nerve cord by a pair of nerves that pass around the esophagus.

  1. Remove the animal's entire digestive tract, and float itinwater. Observe the various

parts, especially the connections of the digestive gland to the stomach.

  1. Cut through the stomach, and noticeinthe anterior region of the stomach wall the

heavy, tooth-like projections, called the gastric mill, which grind up food. Do you see any grinding stones ingested by the crayfish?^ -~^ ,t;.5"' Label the internal anatomy .. lof,N.Wlloilr,U.C

Exercise2:AnatomyofGrasshopper Thegrasshopperis^ aninsect.^ Thepresenceof a^ single^ pair^ of^ antennaeanduniramous appendagesamongother^ features^ identifies^ insectsasbelonging^ to^ theClass^ Hexapoda.^ Like^ the crustaceans,thebody ofinsectsisseparated^ into^ thehead,thorax,and^ theabdomen,^ but^ thereare noappendages^ attachedto^ theabdomen.^ Insectsareadaptedtolifeon^ land.Ininsects^ with^ wings, suchasthegrasshopper,^ wingsareattachedto^ the^ thorax.Respirationis^ bya highly branched internal systemoftubes,calledtracheae^.

External Anatomy

  1. Obtainapreservedgrasshopper,andstudyitsexternal^ anatomy.^ With^ thehelp of^ the Photographic^ Atlas,youwill^ drawtheexteriorandinterior ofthegrasshopperinthe boxesfollowingthedissection^ instructions^ .Makesuretolabelthebolded^ structureson your drawingsasyou go thoughthedissection.

Identifythe three tagmata: head,^ thorax,^ and^ abdomen.

Thegrasshopper 's thorax^ consistsofthree^ fused^ segments:^ the^ large^ anterior

prothorax , themiddle^ rnesothorax,^ and^ the^ hind^ rnetathorax^.^ Identifythefirst pair of

legs attachedto theprothorax^ .Then^ find^ thesecond^ pair^ oflegs^ andthe^ outer^ pair^ of

straight, leathery^ forewings^ attachedto^ themesothorax^.^ Finally^ ,^ locate^ thethird pair of

legs and theinner,^ membranous^ hind^ wings^ attachedto^ themetathorax.^ Each^ leg

consistsoffivesegments.^ The^ hind^ legiswell^ developed^ and^ usedfor jumping. Q: How How many^ pairsoflegs^ arethere?^ C.^ ~(}~ed.^ le5s Q: Is locomotion in thegrasshopper^ adaptedtoland?^ Explain.^ L('{~^ ,^ -Hi-e. Vl~S$

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Spread the wings.^ Notethat^ theydo^ not^ have^ the^ same^ shapeandconsistency (texture) .This^ because^ the^ forewing^ and^ hindwing^ arespecialized^ for different purposes. Thehindwingsare^ used^ for^ flight^ while^ the^ forewings^ are^ usedfor^ balance and stabilization. Useahand lensor dissecting^ microscopetoexamine^ thegrasshopper's^ special^ sense

organs ofthe head.Identifythe^ antennae^ (apair^ oflong,jointed^ feelers),^ the^ compound

eyes, and the dot-like simple^ eyes^ ( ocelli)^.

Remove the mouthparts {palp)^ by^ grasping^ them^ with^ forceps^ and^ pulling^ them^ out.

Arrange theminorderon^ an^ index^ card,^ and^ compare^ them^ to^ the^ diagram^ of^ the^ head and mouth^ partsinthe^ Photographic^ Atlas.^ These^ mouthparts^ are^ used^ for^ chewing^ and are quite different^ from^ those^ of^ a^ piercing^ and^ sucking^ insect.

Identify the tympana (singular,^ tympanum)^ ,^ one^ on^ each^ side^ of^ the^ first^ abdominal

segment.^ The^ grasshopper^ detects^ sound^ vibrations^ with^ these^ membranes.

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~~ ~ ~ ~ ~ ~ ~ ~

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  1. Identify the heart and aorta just beneath the portion of exoskeleton you removed. A grasshopper has an open circulatory system. Remove the heart and adjacent tissues.
  2. Locate the (^) fat body, a yellowish fatty tissue that covers the internal organs. Carefully removeit.
  3. Find the tracheae, the respiratory system of insects. Using the dissecting microscope, look for glistening white tubules, which deliver air to the muscles.
  4. (^) Identify the reproductive organs that lie on either sideofthe digestive tract in the abdomen. If your specimen is a male, look for the testis, a coiled, elongated cord containing many tubules. If your specimen is a female, look for the ovary, essentially a collection of parallel tapering tubules containing cigar-shaped eggs.
  5. Locate the digestive tract and, in sequence, the crop, a large pouch for storing food (a grasshopper eats grasses); the gastric caeca, digestive glands attached to the stomach; the stomach and the intestine, which continues to the anus; and Malpighian tubules, excretory organs attached to the intestine. Insects secrete a solid nitrogenous waste. Is this an adaptation to life on land? Explain. '-{ e5. :::Ih: ~ u-) C>..U. '- +- ux,t\ i:::--~'"v-e S w~ J...l,i.;s c.da.y· <.)
  6. Work the digestive tract free, and move it to one side. Now identify the salivary glands that extend into the thoracic cavity.
  7. Remove the internal organs. Now identify the ventral nerve cord, which is thickened at intervals by ganglia.
  8. Remove one side of the exoskeleton covering the head. Identify the brain, which is anterior to the esophagus.

Identify the internal structures of the grasshopper.

Dorsal Hemocoel artery Crop (^0) varyStomach Heart

Vagina Seminal receptacle Ventral (^) Intestine Oviduct (spermatheca) nerve Ganglia Gastric Malpighian cord (^) caeca tubules (^) c ttay.ic,~MeN.;1. LL c

Identify the mantle, gills, shell, (^) foot, andadductor muscles. Which are part of the visceral mass?

.)

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Activity 3: Squid Dissection

Labialpalp (secre-tes mti1rns tvit1t e11trt1ps food particles)

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The class Cephalopoda includes squids, octopuses, andcuttlefish, among others. These possess beak-like jawsand cansometimes inject poison. The mouthiscentral and surroundedbytentacles. The organism may (^) be up to 17 m long. Octopuses creep andsquids dart via expulsion of water. Cephalopods possess a closed circulatory system (blood is invessels) and well-developed nervous systems with complex brains.

  1. Obtain a squid andlay it dorsal side down (siphon facing (^) up)inthe dissecting tray.
  2. Using a (^) scalpel or dissecting scissors, cut the mantle (^) from its anterior edge (^) next to the siphon to its posterior tip. Donot cut the internal organs.
  3. Using a blunt probe, find the following structures. Label the onesin bold. a. (^) Mantle (^) g. (^) Tentacles b. Siphon (^) h. (^) Ink sac C. Gill i. Chromatophores (small freckle-like spots on the mantle) d. (^) Heart (^) j. (^) Beak (cut the tentacles off close to the eyes to access the beak)

e. Gonad k. Sucker

f. Arms

  1. For a close up of the sucker, cut a 0.5 cm piece of a tentacle and place it on a glass slide. View using the dissecting microscope.

POST-LAB QUESTIONS Name: _____ ______

Comparison of the Crayfish vs. Grasshopper Anatomy, and Clam vs. Squid Anatomy

Crayfish Grasshopper

Locomotion^ __.

-- u:.~^ _.^ cJ^ LA~;^ l'^5 l~~s ·· ~ ~ MrY\ ~1'"" ~ tS (^) - W,~s Respiration

  • &~\ls -^ trac_h:t'.'^ o.~ -S~r~~~ Nervous System _ c~pho.,VzaJi o~ (^) - c~p\·,<d,- zc.J~f\

Reproductive Features _ &o'(_(J) c ha,,-SVY..^ -^ 5~u.o--
r-c p,ei d uc.-h-e _("_ Sensory organs (^) - A--At-0'~ A Vl.--~nna.c CM Pcl<'S Ov"
-~pou.ncl ~tJCS^ ~ o.bdoM.~^ ~~^ I.^ lt:f'c,i^ C)y.^ .-he

-·' ·. Clam. -- -- Squid · Locomotion -u~~ ~'r FceA-- W 'J'f{Jll~

~ ~~+- ~~~J~<?A

Respiration -+hr~'- h,J{} -1-u,bt s -~~~F~ ( s; """~ ')^ 4$~c,.}ef Nervous System (^) , \¼-t---l-- ~ < cf' Cc o.-J'tf,v.. - ( 'QNV.Pl~ v'\efveM.S

~'Q'Z> ~ l-- 43 ' ~ ~o...~~~ Shell structure (^) - C!_ c/l'b ,-Sl> o F ~1 ll.. - ~HI.(_, -+Q',uks

-~'t~ -c=;" -^ C...;^ f'C l<-^ _a( k.l (^) <?<~

Feeding _ Q'" ~ ,e_ c:,wrM .f.s u-.,~.t!.-~ ~~<; Q'lat

mechanism ~ MO.I~ wa:kK ,~ -b J S u c.Aivo, $ u.~~~ ·~ts ~~

v ?Y FY v ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~

~ ~ ~ ~ ~ ~ ~

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