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L3: Lab Exam 3- Requires Respondus LockDown Browser
Due No due date Points 30 Questions 12 Time Limit 60 Minutes Requires Respondus LockDown Browser
Instructions *Note: Lab Notebooks are allowed to be used during the Lab Exam. They must be handwritten or printed off to be utilized due to Lockdown Browser not allowing tabs and files to be open during an exam.
- Attempt History Attempt Time Score LATEST. ; Attempt 1 47 minutes 30 out of 30
Score for this quiz: 30 out of 30
Submitted Apr 24 at 2:45pm
This attempt took 47 minutes. " Question 1 25/25pts | List the four main steps used to prepare a DRY mount, and indicate which step is optional. |
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‘ Your Answer: | : _ & 1 - To clean the slide with a 70% ethanol ‘ ! 2 - (Optional) To draw at the bottom of the cover slide (a circle for the area), of specimen plécement/ location
R e L - fi"-’f Q-1 eine a <terilInnn tn anlv areanism onta the slide: (From cultire and or to-nick from colonies mixine with dishlled water if fram
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2 (Optional) To draw at the bottom of the cover slide (a circle for the area), of specimen placement/location
3- Using a steril loop to aply organism onto the slide: (From culture and or to pick from colonies mixing with distilled water if from a
plate.)
4 - Air dry at room temperature until all the moisture has evaporated pre? N '
1 - Clean slide 2 - Circle area on slide for specimen placement (OPTIONAL) 3 - Apply organism to slide 4 - Air dry at room temperature S i. < ) Question 2»^2 25/25pts When preparing a glass slide for a dry mount, what cleaning solution was used? Your Answer:
70% ethanol or Chem Wipes
70% ethanol Scanned with
Question 5 :^ 2.5/^ 2.5^ pts Why are Gram-positive bacteria able to retain the crystal violet dye? Your Answer: - S It is because of their thick "Peptidoglycan Layer" ~ They contain a thick peptidoglycan Iay;ar in their cell wall that readily retains the dye. e p— Question 6 :^.^ ,^ J^ «^ 25/25pts Identify the stain and shape of the bacteria pictured below. o ¥
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ey I
S 50 ot
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Your Answer:
Gram (+) , Grape like Cluster (Purple)
Gram-positive; Cocci (spherical)
Question 7 :^ _^ 2.5/25pts^ } An acid-fast stain is most commonly used to identify what type of bacterium? What is the name of the primary dye used in this
technique? '
Your Answer:
- To identify Mycobaterium (TB Cultures).
- Carbolfuchsin Dye (red dye) ' Mycobacterium; carbolfuchsin dye | L ) (^) L ek — Scanned with
Question 10 2.5/^ 2.5pts What is the proper way to dispose of all materials used during the lab? Your Answer: In a Biohazard bin/container, and then place in the Auto Clave to be disposed off later G \ o All materials must be place in a biohazardous waste bag and placed into an autoclave for sterilization. Question 11 2.5/25pts What are the Gram status, shape and identification of organism #2 from fhe Gram stain procedure? Your Answer: #2 Escherichia Coli
- Pink Color | Gram Negative | Rod Shape | Like Capsule . ' & ~ | Gram-negative; Bacillus (rod); E. Coli | L ' J Scanned with
TUUI MAILISWECI. #2 Escherichia Coli
- Pink Color | Gram Negative | Rod Shape | Like Capsule A
Gram-negative; Bacillus (rod); E. Coli
Question 12 '^ -^ J^ 2.5/2.5pts What are the Gram status, shape, and identification of organism #4 from the Gram stain procedure? Your Answer: #4 Pseudomonas Aeruginosa
- Pink Color | Gram Negative | Slightly different shape but still qualifies as a Rod Shape | Gram-negative; Bacillus (rod); Pseudomonas
- & Quiz Score: 30 out of 30 < Previous Next » Scanned with
